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kna3@case.edu
Dr. Kumar Alagramam earned his Masters in Biotechnology and Ph.D. in Cell and Molecular Biology from the University of Tennessee at Knoxville . He received postdoctoral training at the Oak Ridge National Laboratory, Oak Ridge, Tennessee, and Case Western Reserve University.
 His research is focused on studying genes associated with development, protection and regeneration of sensory hair cells in the inner ear using the mouse model. Reports from Alagramam's lab provided genetic evidence that mutation in a protocadherin gene causes deafness in mouse and human subjects. One of the aims of his lab is to understand the role of protocadherin genes in the development of sensory epithelia in the inner ear in the mouse model. The other objective of Alagramam's lab is to identify molecular pathways induced or influenced by noise exposure. They are also conducting exploratory research in the mouse whose ultimate aim is to replace damaged hair cells with new hair cells generated from stem cells.
Publications:
1. Alagramam, K. N. , J. Perry, M. Basrai, F. Naider, and J.M. Becker. (1993) Cloning of peptide transport genes from Eucaryotes. Proceedings of t he Thirteenth American Peptide Symposium.
2. Alagramam, K. N. , Naider, F., and Becker, J. M. (1995) A recognition component of the ubiquitin system is required for peptide transport in Saccharomyces cerevisiae. Molecular Microbiology 15(2), p225-234.
3. Woychik, R. P. and Alagramam, K. N. (1998) Insertional mutagenesis in transgenic mice generated by the pronuclear microinjection procedure. Intl. J. Dev. Biol. 42, p1009-1017.
4. Alagramam, K. N ., Kwon, H. K., Cacheiro, N.L.A. Stubbs, L., Wright, G. C., Erway, L. C. and Woychik, R. P. (1999) A New Mouse Insertional Mutation That Causes Sensorineural Deafness and Vestibular Defects. Genetics 152, p1691-1699.
5. Alagramam, K. N. , Zahorsky-Reeves, J., Erway, L. C., Pawlowski, K.S., Wright, C. G. and Woychik, R. P. (2000) Neuroepithelial defects of the inner ear in a new allele of Ames waltzer. Hearing Research 148, p181-191.
6. Alagramam, K. N ., Rohrbaugh, C. L., Kwon, H. K., Pawlowski, K.S., Wright, G. and Woychik, R. P. The mouse Ames waltzer hearing loss mutation is associated with a novel protocadherin gene. Nature Genetics 27, p99-102.
7. Alagramam, K. N ., H. Yuan, M. H. Kuehn, C. L. Murcia, S. Wayne, C. R. Srikumari Srisailpathy, R. B. Lowry, R. Knaus, L. V. Laer, F. P. Bernier, S. Schwartz, C. Lee, C. C. Morton, R. F. Mullins, A. Ramesh, G.V.Camp, G.S. Hagemen, R. P. Woychik, R. J.H. Smith. et. al (2001) Mutations in the novel protocadherin PCDH15 cause Usher syndrome type 1F. Human Molecular Genetics 10, p1709-1718.
8. Hampton , L.L., Cahill, T., Wright, C. G., Alagramam, K. N ., Battey, J. F., K. Noben-Trauth (2003) A new spontaneous mutation in the mouse Ames waltzer gene, Pcdh15. Hearing Research 180(1-2):67-75.
9. Ball, S., Bardenstein, D., and K. N. Alagramam. (2003) Assessment of retinal structure and function in Ames waltzer mice. Invest. Opth. Vis. Sci. 44(9): 3986-3992.
10. Washington, J. W., Davis, R. R., Wright, C. G., Alagramam, K. N. (2005) Characterization of a new ENU allele of Ames Waltzer. Hearing Research 202(1-2):161-169.
11. Adato A, El-Amraoui A, Kikkawa Y, Alagramam K.N. , Weil D, Yonekawa H, El-Amraoui A, Petit C. (2005) Interactions in the Usher syndrome type 1 (USH1) proteins network. Human Molecular Genetics 14(3):347-356.
12. K.N. Alagramam , Stahl, J.S., Jones, J.S., Pawlowski, K. P., C.G. Wright (2005) Characterization Of Vestibular Dysfunction In The Mouse Model For Usher Syndrome 1F. JARO 6(2):106-18.
13. Jones, S.M., Johnson, K.R., Yu, H., Erway, L.C., Alagramam, K.N. Pollak, N. and T. Jones. (2005) A quantitative survey of gr a vity receptor function in mutant mouse strains. JARO 6(4):297-310.
14. Semaan MT, Alagramam KN, Megerian CA. (2005) The basic science of Meniere's disease and endolymphatic hydrops. Curr Opin Otolaryngol Head Neck Surg. 13(5):301-7.
15. Pawlowski, K. P., C.G. Wright, and K.N. Alagramam . (2006) Progression of inner ear pathology in Ames waltzer mice and the role of protocadherin 15 in hair cell development. JARO Jun;7(2):83-94.
16. Pagedar, N., Wang, W., Chen, D., Wright, C.G., Davis, R., and Alagramam, K.N. (2006) Gene Expression Analysis Of Specific Cell Types Isolated From The Mouse Inner Ear Using Laser Capture Microdissection. Brain Res. May 26;1091(1):289-99.
17. Zheng Q. Y., Rozanas, C. R., Isolde T; Mark, C. R., Alagramam, K. N. (2006) Inner Ear Gene Expression and Proteomics of Mouse Models for Deafness, a Discovery Strategy. Brain Res. 1091:113-21.
18. Davis , R. R., Kuo, M-W, Stanton , S., Canlon, B., Krieg, E., Alagramam, K.N. (2006) No Protection from Premature Presbycusis by N-Acetyl Cysteine in C57BL/6J Mice. Hearing Research Aug 21; [Epub ahead of print] PMID: 16930891.
19. Yerukhimovich, MV, Bi, L., Chen, H-C, Miller, RH, Alagramam, K.N . (2006) Identification and Characterization of Mouse Cochlear Stem Cells. Dev. Neuroscience 2006 Oct 17; [Epub ahead of print] PMID: 17047322.
20. K. L. Hall, D. R. Pitts, S. Anne, M. T. Semaan, K.N. Alagramam , C. A. Megerian. (2006) Optimization of RNA Detection from Archival Guinea Pig Temporal Bone Specimens. Otol. Neurotol. Jan;28(1):116-23. PMID: 16983313 .
21. Zheng, QY., H. Yu, J.L. Washington III, L.B. Kisley, K. S. Pawlowski, C.G. Wright, K.N. Alagramam . (2006) A New Spontaneous Mutation in the Mouse Pcdh15 Gene. Hear. Res. 219(1-2):110-20.
22. Anne, S., Kisley, L, Tajuddin, S.T., Leahy, P., Alagramam, K.N. , Megerian, C. A. (2007) Molecular Changes Associated with Endolymphatic Hydrops. Otol. Neurotol. (in press).
23. Alagramam, K. N., Miller , N. D., Adappa, N.A., Pitts, D. R., Heaphy, J. C. Yuan, H., R. J. Smith (2007), Promoter, Alternative splicing and Genomics Structure of Protocadherin 15. Genomics 90, p482-92. Epub 2007 Aug 15.
24.
C.A. Megerian., Semaan, M., Kisley, L., Zheng, Q.Y., Pawlowski, K.S., Wright, C.G., Alagramam, K. N (2008) A Mouse Model with Pathologic Correlates Associated with Meniere's Disease. Hearing Research (In press).
25.
Kikkawa, Y.S., Pawlowski, K.S., Wright, C.G., Alagramam, K.N. (2008) Development of Outer Hair Cells in Normal and Ames Waltzer Mice: Cuticular Plate and Associated Structures. Anatomical Records 291, p224-32.
26. Bixenstine, P.J., Maniglia, M.P., Vasanji, A., Alagramam, K.N. , Megerian, C.A. (2008) Spiral Ganglion Degeneration Patterns in Endolymphatic Hydrops. Laryngoscope (In press). Recent Presentations
Yerukhimovich, M., Bai, L., Chen, D, Miller, R, Alagramam, K.N. Identification and Characterization of Mouse Cochlear Stem Cells 30th Annual Midwinter Research Meeting of the Association for Research in Otolaryngology, Denver , Colorado , USA , February 10-15, 2007.
Zheng QY, Tong Y, Yu H, Li S, Alagramam, K.N. , Identify and Develop Mouse Models for Otitis Media, 30th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology, Denver, Colorado, USA, February 10-15, 2007.
Zheng QY, Yu H, Kisley L, Kikkawa Y, Pawlowski K, Wright C, Alagramam, K,.N. Characterization of Pcdh15av-6J, an Allele of Mouse Protocadherin 15 Gene. 30th Annual MidWinter Research Meeting of the Association for Research in Otolaryngology, Denver , Colorado , USA , February 10-15, 2007.
Lab members
- Daniel Chen - Research Assistant IV
- Joseph Eustache - Research Assistant II
- John Heaphy - Student
- Saba Aftab, PGY3 – Resident
- Ruishuang Geng - Postdoctoral Research Associate
- Chris Heddon - Medical Student
- Alex Levitt – Medical Student
- Bharath Surapaneni - Medical Student
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